|MadSci Network: Biochemistry|
I have recently conducted several experiemnts as part of my coursework. The aim of my investigation is to find out how changing the conc. of H2O2 affects the rate of reaction (immobilized catalase) The enzyme is trapped in beads made from sodium aliginate. My results show that increasing the conc. from 5 volume to 20 volumes of H2O2 has no effect on the rate of reaction. I thought that maybe the reaction was zeroth order with respect to H2O2, but later thought that it may be that I am not using enough enzyme (therefore not enough active sites) for the conc. I selected. Could you tell me what should happen? and why I obtained the results I did?
Re: How does conc. of Hydrogen Peroxide affect the IMMOBILIZED enzyme catalase?
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