Growing bacteria..

The following answers discuss how to grow different kinds of bacteria. A variety of microbiology laboratory manuals go into more detail.

Links in the reagents/suppliers section of the MadSci Library indicate where microbiological media and supplies may be purchased.

The Micro Science Fair help pages has additional information, with descriptions, regarding specific techniques, equipment and reagents.

Strict safety precautions should be followed when growing bacteria, whether a known strain, or unidentified species from environmental sources. Pure cultures of otherwise "harmless bacteria" can become contaminated with potential pathogens. Likewise, bacteria cultured from the skin, mouth, sinks or dirt can be potentially dangerous if they get into cuts or in contact with mucous membranes including the eyes or mouth. Always wear gloves (preferably latex) and protective clothing (a lab coat and safety goggles) when handling materials and be certain to properly dispose of your experiments when finished.

We encourage you to discuss projects involving the growth of bacteria with your science teacher to make certain appropriate facilities for the growth and handling of microorganisms are made available to you.

Questions involving culturing of bacteria.
What are some accurate labratory techniques obtaining normal skin flora?
Topics for a microbiology lab?
Effects of peppermint on bacteria
How to streak bacteria onto agar plates
Incubating agar plates
How can I make an incubator?
Freezing bacterial cells
What is the best way to store E. coli?
Counting bacteria with 10-fold dilutions. (Dilution curve).
How do I stop moisture from spreading bacteria on agar plates stored upside down?

Microbiological media
Are there vegetable and meat based agars made for culturing E. coli?
Gelatin agar?
Growing bacteria (blood agar)
Brain Heart Infusion Agar
What is the red medium in Petrie dishes?

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