Why is agarose used in electrophoresis instead of agar ?

Subject: Why is agarose used in electrophoresis instead of agar ?

Date: Sun Feb 9 05:08:05 2014
Posted by Tushar
Grade level: undergrad School: Indian Institute of Science
City: Bangalore State/Province: Karnataka Country: India
Area of science: Molecular Biology
ID: 1391947685.Mb

Message:


As agarose is nothing but agar, with only agaropectin removed from it, I was 
wondering why do we not use Agar for electrophoresis instead of agarose ? 
Furthermore, how does addition of water instead of buffer ( which only acts to 
adjust the pH) lead to melting of casted gel in electrophoresis ?

Re: Why is agarose used in electrophoresis instead of agar ?

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