To precipitate DNA can I use sodium acetate ibstead of ammonium acetate?

Subject: To precipitate DNA can I use sodium acetate ibstead of ammonium acetate?

Date: Tue Mar 22 14:25:23 2005
Posted by Clara
Grade level: grad (science) School: Department of Zoology, University of Coimbra
City: Coimbra State/Province: No state entered. Country: Portugal
Area of science: Molecular Biology
ID: 1111523123.Mb

Message:


I have a DNA extraction protocol that uses ammonium acetate 7.5M. I was told 
that instead I could use the same amount of sodium acetate at the same 
concentration. I found it very hard to make a solution 7.5M of sodium acetate. 
I check several different protocols and they all use a 3M solution. I would 
like to know at what concentration and the amount of sodium acetate I should 
use instead of ammonium acetate 7.5M

Re: To precipitate DNA can I use sodium acetate ibstead of ammonium acetate?

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