MadSci Network: Cell Biology |
Hey Stephanie –
This is a
really interesting
question – as I write this response I am suffering from a nasty case of the
flu, so I am especially interested in microbicides! Your question is interesting because
people
are instructed by many health officials to dilute bleach by a factor of ten
before using it. We use this in our
own
lab to disinfect surfaces, especially surfaces that might otherwise be
damaged
by undiluted bleach. It is worth
noting
that the high pH of undiluted bleach also ensures that the antimicrobial
component of bleach, sodium
hypochlorite, remains stable. At
lower pH it can break down into hypochlorite ions and chloramines.
A group English
of
scientists made several dilutions of sodium hypochloriteand
tested their efficacy in killing the yeast Candida albicans and the bacterium Enterococcus
faecalis.
They
found that solution strengths from 5.25% to 0.5% were very effective at
killing
yeast. However, E. faecalis
was a little more resilient. At
5.25%
(the concentration of undiluted household bleach), 79% of bacteria were
killed
after 30 seconds exposure. However,
at
0.5% (so…roughly 1:10 dilution) only 17% were killed after 30 seconds
exposure.
So, all things
being equal, undiluted bleach is more effective at killing stubborn
bacteria. It is important to note,
however, that after 30 minutes of exposure, 5.25% and 0.5% were equally
effective at killing bacteria – 100% of them, in fact.
The data of
viruses
is a little more straight forward – viruses are simply not cut out to
defend
against the bleach onslaught! A
group of
scientists in New York studied the effects of very dilute concentrations of
bleach on the
respiratory
syncytial virus (RSV). A tenfold
dilution of bleach, which subsequently mixed with an equal volume of
RSV-containing medium (so in fact a twentyfold dilution) eradicated all of the virus.
A 100-fold
dilution of bleach killed 100% of the virus half of the time, and decreased
the
number of live viral particles by greater than three logs in the other half
of
the tests. This was all after five
minutes of treatment.
So at least for
viruses, you can probably dilute the bleach tenfold without worrying too
much
about decreased antimicrobial activity.
I hope this
helps
with your studies – I’m still going to dilute my bleach because I can’t
handle
the smell!
Regards,
Billy.
Check out these
papers for more information:
Radcliffe CE,
Potouridou L, Qureshi R,
Habahbeh N, Qualtrough A,
Worthington H, and Drucker D. “Antimicrobial activity
of
varying concentrations of sodium hypochlorite on the endodontic
microorganisms Actinomyces israelii, A.
naeslundii, Candida albicans,
and
Enterococcus faecalis.” International
Endodontic
Journal. 37(7). 2004.
PMID: 15189432
Krilov L and
Harkness M. “Inactivation of
respiratory
syncytial virus by detergents and disinfectants.” Pediatric Infectious
Disease Journal. 12(7). 1993. PMID: 8346001
This Oregon
State
University Research Office publication also contains some interesting
information about the chemical qualities of bleach:
“Fact Sheet:
Disinfection Using Chlorine Bleach.” Oregon State
University
Research Office. Published December, 2011.
Accessed January, 2013.
http://oregonstate.edu/dept/larc//sites/default/files/pdf/chlori
ne-fact-sheet.pdf
Try the links in the MadSci Library for more information on Cell Biology.